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KMID : 0368420200630030165
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Journal of Plant Biology
2020 Volume.63 No. 3 p.165 ~ p.175
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N-Glycoproteome Reveals That N-Glycosylation Plays Crucial Roles in Photosynthesis and Carbon Metabolism in Young Rice Leaves
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Wang Jiafeng
Wen Hui
Li Ming
Guo Tao
Chen Chun
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Abstract
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N-Glycosylation, one of the most prominent and abundant protein post-translational modifications in eukaryotic cells, is involved in diverse biological processes. To date, large-scale profiling of the N-glycoproteome has been only reported in rice germinating embryos, but that in rice leaves has not been profiled. Here, we report the first rice N-glycoproteome in leaves, determined by combining Concanavalin A (ConA) lectin affinity chromatography enrichment and high-resolution LC?MS/MS. In total, 282 N-glycopeptides, corresponding to 556 proteins and 643 sites, were identified from the leaves of H4 (indica) and LTH (japonica). Two conserved canonical N-glycosylation motifs N-X-T and N-X-S and two more non-canonical motifs N-X-S-X-N and A-X-X-N-X-S were revealed in rice. More than 50% of the identified proteins are localized to the chloroplast, extracellular part, and plasma membrane. Bioinformatics analysis revealed that N-glycosylation occurs on proteins involved in a wide variety of biological processes, especially photosynthesis and carbon metabolism. Protein?protein interaction networks of these proteins provided further evidence that N-glycosylation contributes to a wide range of regulatory functions. In summary, these findings revealed the complexity of the rice N-glycoproteome and provided useful information to further explore the regulatory roles of N-glycosylation in the growth, development, and stress responses of rice.
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KEYWORD
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N-Glycosylation, N-Glycoproteome, Oryza sativa L., LC?MS/MS
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